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At the Institute of Pathology
Department of Molecular Pathology
Klinikum Stuttgart
Kriegsbergstr. 60
70174 Stuttgart
(located 500 m from the railway station, 15 min by subway from Stuttgart airport, liable parking available 200 m apart from the conference site)
The HOPE-fixation technique invented by Dr. Jürgen Olert and further developed in collaboration with Dr. K.H. Wiedorn, Prof. Vollmer and some other researchers has now shown its remarkable impact regarding the enhancement in immunohistochemistry and molecular pathology.
Although the HOPE-fixation technique offers great advantages in comparison to formalin fixation the use of the HOPE-fixation technique has been confined to few research labs because of the lack of an automatic embedding system capable of handling a large probe set simultaneously.
During this workshop the HOPE-fixation technique will be described in detail and downstream applications handling with HOPE fixed tissues (conventional staining with HE and PAS, immunohistochemistry, PCR, lasermicrodissection, tissue microarrays, DNA MicroArray Analysis, Sequencing, K-ras Mutation screening ) will be demonstrated in practical workshops.
Furthermore participants will have the opportunity to use the new hope embedding automat HS-100.
Speakers:
Prof. Dr. med. Alexander Bosse (Institut für Pathologie, Klinikum Stuttgart)
Dr. med. Klaus Hermann Wiedorn (Institut für Pathologie, Klinikum Stuttgart)
Dr. rer. nat. Dieter Techel (Institut für Pathologie, Klinikum Stuttgart)
Dipl. Biol. Stefan Jenner (Institut für Pathologie, Klinikum Stuttgart)
Hans Georg Siek (VIP, Linden)
Gerhard Zimmerman (Leica, Bensheim)
Dr. Ralf Greim (DCS, Hamburg)
Dipl. Biol. Slavica Dimitrovska (DCS, Hamburg)
Dr. Christof Steiner (Digital Biomedical Imaging Systems AG, Pforzheim)
Dr. Jörg Stappert (Greiner BioOne, Frickenhausen)
Udo Schimmel (Alphametrix, Rodgau )
Technical assistance:
Maria-Reyes Esparza (Institut für Pathologie, Klinikum Stuttgart)
Jutta Matthus (Institut für Pathologie, Klinikum Stuttgart)
Jianping, Gao (Institut für Pathologie, Klinikum Stuttgart)
| Klick here for the position plan -> (open in new window) |
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Speakers:
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Prof. Dr. med. A. Bosse (Pathologie, Klinikum Stuttgart) |
Dr. med. K.H. Wiedorn (Pathologie, Klinikum Stuttgart) |
Dr. Ralf Greim (DCS) |
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H.G. Siek (VIP) |
U. Schimmel (Alphametrix) |
G. Zimmerman (Leica) |
Technical assistance:
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Jutta Matthus (Pathologie, Klinikum Stuttgart) |
Maria-Reyes Esparza (Pathologie, Klinikum Stuttgart) |
Program:
24.04.08 Thursday
12:00-12.15 Prof. Dr. A. Bosse Opening and Introduction
12:15-14:15 Dr. K.H. Wiedorn Overview on applications for the HOPE-technique
14:15-14:30 coffee break
14:30 – 16:00 Dr.K.H. Wiedorn, Mrs Matthus
Practical Workshop: Cutting and mounting slices from HOPE-fixed tissue and HE staining
16:00 – 16:30 H.G. Siek, Dr. K. H. Wiedorn
The HOPE-Tissue Processing System HS-100
16:30-16:45 coffee break
16:45-17:45 Dr. K. H. Wiedorn, H.G. Siek, Mrs Matthus
Practical Workshop: Automatic HOPE embedding using the HS-100 automat (Part 1)
17:45-18:15 G. Zimmermann
Laser Mikro Dissection, technical features and applications
18:15-19:45 G. Zimmermann, Dr. K. H. Wiedorn
Practical Workshop: Lasermicrodissection
25.04.08 Friday
8:30-10:00 Dr. K. H. Wiedorn, Mr Matthus, Mr Gao
Nucleic acid extraction using magnetic beads technology on the KINGFISHER ml (Part 1)
10:00 – 10:45 H.G. Siek, Dr. K. H. Wiedorn, Mrs Matthus
Practical Workshop: Automatic HOPE embedding using the HS-100 automat (Part 2)
10:45-11:00 coffee break
11:00-12:00 Dr. K. H. Wiedorn, Mrs Matthus, Mr Gao
Practical Workshop: Nucleic acid extraction using magnetic beads technology on the KINGFISHER ml (Part 2)
12:00-13:30 Dr. K. H. Wiedorn, Mr. Gao
Practical Workshop: PCR Set up using the CAS1200
13:30-14:30 lunch break
14:30 – 15:15 Dr. D. Techel
Sequencing
Dr. Ralf Greim, Dipl. Biol. Slavica Dimitrovska
15:15 – 16:30 Practical Workshop Immunohistochemistry on HOPE fixed tissues (Part 1)
16:30 – 17:15 Oral presentation HOPE and immunohistochemistry
17:15 – 17:30 coffee break
17:30 – 19:30 Practical Workshop Immunohistochemistry on HOPE fixed tissues (Part 2)
26.04.08 Saturday
9:00-9:30 Dr. Christof Steiner
MArS Microarray Scanner - the Affordable Solution for Biochip Applications in Diagnostics
9:30 – 10:00 Dr. J. Stappert
PapilloCheck®, microarray based HPV screening test for clinical diagnostics
10:00 – 11:00 Dr. K.H. Wiedorn, Dr. J. Stappert
HPV Typing using DNA MicroArray Analysis Practical Workshop (Part 1)
11:00 – 11:15 Coffee Break
11:15 – 11:45 U. Schimmel
Agilent 2100 Bioanalyzer: One Platform for DNA/RNA/Protein and Cell Analysis
11:45 – 12:45 Dr. K.H. Wiedorn, U. Schimmel
Practical Workshop: gel electrophoresis (Part 1)
12:45 – 13:30 Lunch Break
13:30 – 15:30 U. Schimmel
Practical Workshop: Construction and automated analysis of tissue microarrays with HOPE
15:30-15:45 coffee break
15:45 – 16:15 Dr. K.H. Wiedorn, U. Schimmel
Practical Workshop: gel electrophoresis (Part 2)
16:15 – 16:45 Dr. K.H. Wiedorn, Dr. J. Stappert
HPV Typing using DNA MicroArray Analysis Practical Workshop (Part 2)
16:45 – 17:30 U. Schimmel
Transcriptome Profiling and Analysis of Gene Regulation by Highly Flexible Microarrays
Registration Form
I herewith register for the HOPE Workshop 24.04.08 – 26.04.08 at the Institute of Pathology, Klinikum Stuttgart.
Fax to: +49-711-278404917 or mail to k.wiedorn@katharinenhospital.de
Name:
Titel:
Institution:
Address:
Fon:
Fax:
E-Mail:
Date: Signature:
As only a limited number of interested can participate in the workshop places will be reserved on first come first serve basis.
A fee of 185€ has to be payed in advance after you have received the notification of acceptance for the workshop.